DIAGNOSIS OF CHLAMYDOPHILA ABORTUS BY SPECIES –SPECIFIC ELISA TECHNIQUE WITH REAL TIME PCR CONFIRMATION IN CAMELS AND SMALL RUMINANTS IN THE WEST-NORTHERN DESERTOF EGYPT.

Serological surveys for antibodies of Chlamydophilaabortusin camels and small ruminants in the northern-west coast of Egypt were carried out from August 2014 to January 2015; some cases had a history of abortion. A total of 630 blood samples were randomly collected from the jugular vein of camels, sheep and goat. Camels were 228 pregnant female and 48 male with average ages 5:12 years, 224 Ewes and 130 goats with age average 3: 5 years old with or without apparent clinical symptoms. One hundred and eighty different swabs (four swabs from each animal were taken fecal, vaginal, nasal and ocular swabs) were collected from 45 female camels, 224 swabs from 56 ewes and 52 swabs from 13 goats and were subjected to serological analysis by IDEXX Enzyme Linked Immunosorbent assay using an ELISA kit (IDEXX Swizerland). All male camel samples were negative while female samples expressed Chlamydophilaabortus antibodies with positive percent S/P% equal to 10.87%(30/276). The sero-prevalencein ewes was observed in 3.57% (8/224), whilein goats was 15.38% (20/130). Goats have recorded higher antibody titers than ewes, there was a significant relation between age and disease in camels and goats at 0.01 level, and in sheep at 0.05 level. DNA were extracted from swabs of the positive serology animals. Quantification of chlamydiaceae by real time PCR were 81.2% positive in camels,100% in ewes and 50% in goats.