MARKERS OF ATHEROGENICITY AND INFLAMMATION IN H.PYLORI INFECTED PEPTIC ULCER PATIENT

Peptic ulcer has been implicated in the pathogenesis of some non-gastric pathology, especially in cardiovascular disease. This study is aimed at determining the levels of markers of atherogenecity and inflammation in H. pylori infected peptic ulcer patients. A total of 60 subjects were recruited for the study of which 40 were subjects with peptic ulcer (20 are peptic ulcer patients with H.pylori and 20 are peptic ulcer patients without H. pylori) while 20 were healthy subjects. Five (5mls) of fresh venous blood was collected from all the participants by venepuncture using sterile needle and syringe. It was dispensed into clean plain sample container, and allowed to cloth and retract. The serum were separated into plain containers and stored at -20°c prior to use. All reagents were commercially purchased and the manufacturer’s operational instructions were strictly followed. Lipid profile, malondialdehyde and C-reactive protein was determined using the following procedures. Lipid profile was determined using enzymatic method, malondialdehyde was determined using enzymatic method while C-reactive protein was determined using ELISA method. All values were expressed as mean ± standard deviation. The statistical analysis was carried out using student’s t-test. Values with level of significance (p<0.05) was considered to be statistically significant. There was a significant increase (p=0.002, p=0.005, p=0.000, and p=0.000 respectively) in the mean value of total cholesterol (181.73± 16.50)mg/dl, triglyceride (114.04±19.07)mg/dl, MDA (3.63±0.58)mmol/L and C-reactive protein (4.32±0.97)ug/ml in Peptic Ulcer Patients without H.pylori when compared to controls (152.00±13.90)mg/dl, (82.76±16.50)mg/dl, (2.14±0.32)mg/dl and (1.78±0.37)ug/ml respectively. The mean value of HDL was significantly reduced (p=0.000) in Peptic Ulcer Patients without H.pylori (36.93±7.25)mg/dl when compared to control (55.46+ 4.94)mg/dl. There was a significant increase (p=0.001, p=0.003, p=0.000, and p=0.000 respectively) in the mean value of total cholesterol (241.14±44.90)mg/dl, triglyceride (136.36±30.65)mg/dl, MDA (4.71±0.54)mmol/L and C-reactive protein (6.09±1.03)ug/ml in Peptic Ulcer Patients with H.pylori when compared to controls (152.00±13.90)mg/dl, (82.76±16.50)mg/dl, (2.14±0.32)mg/dl and (1.78±0.37)ug/ml respectively. The mean values of HDL was significantly reduced (p=0.000) in Peptic Ulcer Patients with H. pylori (31.32± 5.96)mg/dl when compared to control (55.46±4.94)mg/dl. There was a significant increase (p=0.007, p=0.003 and p=0.006 respectively) in the mean value of total cholesterol (241.14± 44.90)mg/dl, MDA (4.71±0.54)mmol/L and C-reactive protein (6.09±1.03)ug/ml in peptic ulcer patients with H.pylori when compared with Peptic Ulcer Patients without H. pylori (181.73±16.50)mg/dl, (3.63±0.58)mmol/L and (4.32±0.97)ug/ml respectively. There was no significant difference (p=0.128 and p=0.140 respectively) in the mean value of triglyceride (136.36±30.65)mg/dl and HDL (31.32±5.96)mg/dl in Peptic Ulcer Patients with H.pylori infectionwhen compared to Peptic Ulcer Patients with H.pylori infection (114.04±19.07)mg/dl and (36.93±7.25)mg/dl respectively Though there was some differences in the individual results. H. pylori infected peptic ulcer is associated with increased level of total cholesterol, triglyceride, malondialdehyde and C-reactive protein and a decreased level of HDL. From these results, there is a clear indication of inflammation and dyslipidaemia, which suggest acute association of H.pylori infection with cardiovascular diseases in peptic ulcer patients.