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Calcium hydroxide the most commonly used intracanalmedicament, should be completely removed from root canal walls before obturation, otherwise it may hinder the penetration of root canal sealers and affect the proper fluid tight seal. Canal shape and method of applicationhad an impact on the residual calcium hydroxide remaining. Application with a lentulo spiral has been found to be more homogenous and deeper in tubules than injection of Ca(OH)2 paste. Different techniques have been introduced to improve removing of calcium hydroxide from root canal system. But till date none of the techniques was able to completely remove calcium hydroxide. The aim of this study was to compare the cleaning efficacy of endoactivator, Canal brush and ProUltrain removing calcium hydroxide from root canal walls.