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Indigofera linnaei, Ali, belongs to the family Fabaceae, is a medicinal plant growing wild in tropical countries.In the present investigation, the aerial leaf sections of Indigofera linnaei were examined for Triterpenoids, steroids, saponins, tannins, phenols, flavonoids, alkaloids, glycosides and coumarins were found in the ethanol and aqueous extracts of the leaves of Indigofera linnaei. The quantitative estimation of phytoconstituents in the powdered samples of Indigofera linnaei revealed that flavonoids were found in significant quantities when compared to alkaloids, tannins, phenols, and saponins. To evaluate the antimicrobial activity of ethanol extract using the standard disc diffusion method against three organisms. The antibacterial activities of the ethanolic extracts were compared favorably with that standard bacterial antibiotic (Chloramphenicol) and fungal antibiotic (Fluconazole). The Ethanolic extract of leaf showed a maximum zone of inhibition in Escherichia coli(7.65±0.53) againstStaphylococcus aureus(4.71±0.32)andCandida albicans (5.12±0.35).