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Recent development in nanotechnology makes nanomaterial a promising agent with wide range of applications in major fields like medical, agriculture, bio-remediations, industries etc. Green synthesis of nanoparticle using plant and microbes is considered to be safe and environment friendly approach. Among various nanoparticles metal nanoparticle gain more attention due to their stability, catalytic abilities and applicability’s. Diverse group of bacteria and plants are being employed for synthesis of metal nanoparticles. Characterization tool such as SEM, TEM, XRD, FTIR, DLS, AFM reveals morphology of synthesised particles. This review gives overall summary of various plants and bacteria mediated approaches used to synthesize metal nanoparticles, morphology and applications of synthesized particles. This Research would also include useful findings related to comparison between plants and bacteria mediated nanoparticles in terms of Morphology, size distributions and applications in various fields.