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The human scalp is susceptible to microbial build-up if not thoroughly and frequently washed with the appropriate cleansing agent such as shampoo. Shampoo is a fast-moving commodity for hair care that is used to get rid of contaminants such as dandruff, oils, grime, skin fragments, and other contaminants that slowly accumulate in the hair. The purpose of shampoo is to remove the undesirable buildup without extracting too much sebum to leave hair unmanageable. The chemicals in shampoo may harm the healthy microorganisms in our hair. In this study, the antibacterial efficacy of the most popular shampoo products is investigated. In order to evaluate the quality in terms of biocampatibility four different brands of shampoo for both regular shampoo and anti-dandruff shampoo were selected. The present study focussed on In-vitro antibacterial activity of commonly used shampoos. The samples selected were namely Himalaya, Meera, Dove, Pantene. They were examined for in-vitro antibacterial activity for bio-compatibility. The compound were analyzed for five gram positive bacterias such as Staphylococcus aureus, Staphylococcus epidermidis, Bacillus subtilis, Cutibacterium, Candida albicans, gram negative bacteria Salmonella typhi and One fungi species named as Microsporum canis. The current study highlighted the anti-microbial assay of the commercially available shampoo products based on the well diffusion method. The biocompatibility of the products with seven different microbes were assessed. The experiment results have brought Interesting insights and helped to identify the safe products for dermatological relevance.