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In this study, Khanh Hoa swiftlet nest was hydrolyzed by protamex and bromelain enzyme to prepare new food product lines. The factors affecting the polypeptide hydrolysis process were investigated to find out optimum hydrolysis conditions for the two enzymes. Bromelain showed higher hydrolysis activity than protamex in the investigated conditions. The optimum hydrolysis conditions for 1% swiftlet nest solution were 0,5 % enzyme concentration at 600C in 3h for bromelain enzyme and 0.7 % enzyme concentration at 550C in 4h for protamex enzyme. Compared to swiftlet nest solution, the hydrolysates have higher contents of soluble proteins and free amino acids, which are more antioxidant active and easier for body to absorb.